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FAQ regarding differences between Pfu and Taq DNA polymerases.
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Rapid and Simple Method to Prepare Functional Pfu DNA. – Key words: Pfu, periplasm, expression, osmotic. The DNA polymerase from Pyrococcus furiosus (Pfu) shows the lowest error rate among the thermostable DNA.
Figure 3. Circular assembly amplification for the synthesis of Pfu DNA polymerase. Our circular assembly amplification method uses three tiers of selection to reduce gene synthesis error rates by at least a factor of 7 compared to the.
Construction, Expression, and Characterization of. – Construction, Expression, and Characterization of Recombinant Pfu DNA Polymerase in Escherichia coli
Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division.
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A. General PCR Conditions with Pfu DNA Polymerase 1. Set up the appropriate. D. Error Rates of Pfu, Pwo, Vent, and Taq DNA Polymerases 1. The above.
Pfu Ultra DNA Polymerases & PCR Master Mixes. Pfu Ultra DNA Polymerases & PCR Master Mixes. Pfu exhibits the lowest error rate of any thermostable DNA polymerase ;
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Full Text (PDF) – Of the enzymes characterized to date, Pyrococcus furiosus (Pfu) DNA polymerase exhibits the highest fidelity, with an average error rate estimated to be 2- to.
10× Cloned Pfu buffere 1 ml 2 × 1 ml 4 × 1 ml 20 × 1 ml. the error rate of Pfu DNA polymerase can be avoided by maintaining the